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lr clonase reaction kit  (Thermo Fisher)


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    Thermo Fisher lr clonase reaction kit
    Lr Clonase Reaction Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lr+clonase+reaction+kit/gateway+lr+clonase+ii+enzyme+mix/10__1038_slash_s44318___024___00356___2-373-26-30
    Average 90 stars, based on 1 article reviews
    lr clonase reaction kit - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Amplification:

    Article Title: The Natural Antisense Transcript <i>DONE40</i> Derived from the lncRNA <i>ENOD40</i> Locus Interacts with SET Domain Protein ASHR3 During Inception of Symbiosis in <i>Arachis hypogaea</i>
    Article Snippet: .. Amplified fragments were cloned into pENTR/D-TOPOR (Life Technologies) and then into binary vectors pK7GWIWG2D(II) (Karimi et al. 2002) by gateway technology through the LR clonase reaction kit (Life Technologies). ..

    Article Title: The Natural Antisense Transcript <i>DONE40</i> Derived from the lncRNA <i>ENOD40</i> Locus Interacts with SET Domain Protein ASHR3 During Inception of Symbiosis in <i>Arachis hypogaea</i>
    Article Snippet: .. Amplified fragments were cloned into pENTR/D-TOPOR (Life Technologies) and then into binary vectors pK7GWF2 (Karimi et al. 2002) by gateway technology through the LR Clonase reaction kit (Life Technologies). .. Constructs are then transformed in Agrobacterium rhizogenes strain R1000.

    Clone Assay:

    Article Title: The Natural Antisense Transcript <i>DONE40</i> Derived from the lncRNA <i>ENOD40</i> Locus Interacts with SET Domain Protein ASHR3 During Inception of Symbiosis in <i>Arachis hypogaea</i>
    Article Snippet: .. Amplified fragments were cloned into pENTR/D-TOPOR (Life Technologies) and then into binary vectors pK7GWIWG2D(II) (Karimi et al. 2002) by gateway technology through the LR clonase reaction kit (Life Technologies). ..

    Article Title: Sestrin2 drives ER-phagy in response to protein misfolding
    Article Snippet: The mKeima-LDHB plasmid was a gift from Stefano Santaguida. mKeima-COX8 was purchased from Addgene (Plasmid #131626). .. The plasmids mKeima-FAM134B and mKeima-FAM134C were cloned into the pDONR223 vector (Invitrogen) using the BP Clonase Reaction Kit (Invitrogen, #11789020) and further re-combined, through the LR Clonase Reaction Kit (Invitrogen, #11791020), into the GATEWAY destination vectors iTAP MSCV-N-FLAG-HA IRES-PURO. .. The mutant COL1A2 G610C and COL2A1 R789C plasmids lacking the N-terminal signal peptide (COL1A2 G610CΔER and COL2A1 R789CΔER) were generated by site-directed mutagenesis using the Agilent QuikChange XL Site-Directed mutagenesis kit using the mCHERRY-PC2 and eGFP-PC1 backbone.

    Article Title: The Natural Antisense Transcript <i>DONE40</i> Derived from the lncRNA <i>ENOD40</i> Locus Interacts with SET Domain Protein ASHR3 During Inception of Symbiosis in <i>Arachis hypogaea</i>
    Article Snippet: .. Amplified fragments were cloned into pENTR/D-TOPOR (Life Technologies) and then into binary vectors pK7GWF2 (Karimi et al. 2002) by gateway technology through the LR Clonase reaction kit (Life Technologies). .. Constructs are then transformed in Agrobacterium rhizogenes strain R1000.

    Article Title: Two FAM134B isoforms differentially regulate ER dynamics during myogenesis
    Article Snippet: cDNAs were cloned into lentiviral vectors using GATEWAY (Thermo Fisher Scientific) or In-Fusion (Takara Bio) systems. .. For the GATEWAY cloning the cDNAs were cloned into the pDONR223 vector using the BP Clonase Reaction Kit (Thermo Fisher Scientific) and further recombined, though the LR Clonase Reaction Kit (Thermo Fisher Scientific), into GATEWAY lentiviral destination vectors: pLTD-N-HA-PUROMYCIN lentiviral vector. .. We designed pLTD-N-HA-BLASTICIDIN and pLTD-N-HAHYGROMYCIN lentiviral vectors that were generated by Vector Builder.

    Article Title: Role of FAM134 paralogues in endoplasmic reticulum remodeling, ER‐phagy, and Collagen quality control
    Article Snippet: .. cDNAs were cloned into pDONR223 vector using the BP Clonase Reaction Kit (Invitrogen) and further recombined, though the LR Clonase Reaction Kit (Invitrogen), into GATEWAY destination vectors: iTAP MSCV‐N‐FLAG‐HA IRES‐PURO, pBABE destination vector (Addgene #51070) and pBABE (Addgene #51070) where we enzymatically changed the resistance from puromycin to hygromycin. cDNA mutations were generated via PCR site‐directed mutagenesis according to standard protocols. ..

    Article Title: Phosphorylation of FAM134C by CK2 controls starvation-induced ER-phagy
    Article Snippet: Tamoxifen (Sigma-Aldrich, #H6278) was used at 1 μM for 72 hours. .. To generate HA- and Myc-FAM134s plasmids, FAM134s and FAM134C mutants were cloned into pDONR223 vector (Invitrogen) using the BP Clonase Reaction Kit (Invitrogen, #11789020) and further recombined, through the LR Clonase Reaction Kit (Invitrogen, #11791020), into the GATEWAY destination vectors iTAP MSCV-N-FLAG-HA IRES-PURO or Myc-pcDNA3.1 (table S8). ..

    Article Title: Heteromeric clusters of ubiquitinated ER-shaping proteins drive ER-phagy
    Article Snippet: .. Plasmids are presented in Supplementary Table . cDNAs were cloned into the pDONR223 vector using a BP Clonase Reaction kit (Invitrogen, 11789100) and further recombined into the Gateway destination vectors pcDNA5-FRT/TO-N-mCherry-EGFP, pcDNA3.1-N-HA, pHAGE-GFP, pcDNA3.1-N-Flag, pcDNA3.1-C-Flag, pcDNA3.1-N-SBP-Flag, pGEX6-GST, and the biomolecular complementation affinity purification system vectors pDEST-V1-ORF, pDEST-V2-ORF, pDEST-ORF-V1 and pDEST-ORF-V2 using a LR Clonase Reaction kit (Invitrogen). ..

    Article Title: Heteromeric clusters of ubiquitinated ER-shaping proteins drive ER-phagy.
    Article Snippet: .. Plasmids are presented in Supplementary Table 1. cDNAs were cloned into the pDONR223 vector using a BP Clonase Reaction kit (Invitrogen, 11789100) and further recombined into the Gateway destination vectors pcDNA5-FRT/TO-N-mCherry-EGFP, pcDNA3.1-N-HA, pHAGE-GFP, pcDNA3.1-N-Flag, pcDNA3.1-C-Flag, pcDNA3.1-N-SBP-Flag, pGEX6-GST, and the biomolecular complementation affinity purification system vectors pDEST-V1-ORF, pDEST-V2-ORF, pDEST-ORF-V1 and pDEST-ORF-V2 using a LR Clonase Reaction kit (Invitrogen). ..

    Plasmid Preparation:

    Article Title: Sestrin2 drives ER-phagy in response to protein misfolding
    Article Snippet: The mKeima-LDHB plasmid was a gift from Stefano Santaguida. mKeima-COX8 was purchased from Addgene (Plasmid #131626). .. The plasmids mKeima-FAM134B and mKeima-FAM134C were cloned into the pDONR223 vector (Invitrogen) using the BP Clonase Reaction Kit (Invitrogen, #11789020) and further re-combined, through the LR Clonase Reaction Kit (Invitrogen, #11791020), into the GATEWAY destination vectors iTAP MSCV-N-FLAG-HA IRES-PURO. .. The mutant COL1A2 G610C and COL2A1 R789C plasmids lacking the N-terminal signal peptide (COL1A2 G610CΔER and COL2A1 R789CΔER) were generated by site-directed mutagenesis using the Agilent QuikChange XL Site-Directed mutagenesis kit using the mCHERRY-PC2 and eGFP-PC1 backbone.

    Article Title: Role of FAM134 paralogues in endoplasmic reticulum remodeling, ER‐phagy, and Collagen quality control
    Article Snippet: .. cDNAs were cloned into pDONR223 vector using the BP Clonase Reaction Kit (Invitrogen) and further recombined, though the LR Clonase Reaction Kit (Invitrogen), into GATEWAY destination vectors: iTAP MSCV‐N‐FLAG‐HA IRES‐PURO, pBABE destination vector (Addgene #51070) and pBABE (Addgene #51070) where we enzymatically changed the resistance from puromycin to hygromycin. cDNA mutations were generated via PCR site‐directed mutagenesis according to standard protocols. ..

    Article Title: Phosphorylation of FAM134C by CK2 controls starvation-induced ER-phagy
    Article Snippet: Tamoxifen (Sigma-Aldrich, #H6278) was used at 1 μM for 72 hours. .. To generate HA- and Myc-FAM134s plasmids, FAM134s and FAM134C mutants were cloned into pDONR223 vector (Invitrogen) using the BP Clonase Reaction Kit (Invitrogen, #11789020) and further recombined, through the LR Clonase Reaction Kit (Invitrogen, #11791020), into the GATEWAY destination vectors iTAP MSCV-N-FLAG-HA IRES-PURO or Myc-pcDNA3.1 (table S8). ..

    Article Title: Heteromeric clusters of ubiquitinated ER-shaping proteins drive ER-phagy
    Article Snippet: .. Plasmids are presented in Supplementary Table . cDNAs were cloned into the pDONR223 vector using a BP Clonase Reaction kit (Invitrogen, 11789100) and further recombined into the Gateway destination vectors pcDNA5-FRT/TO-N-mCherry-EGFP, pcDNA3.1-N-HA, pHAGE-GFP, pcDNA3.1-N-Flag, pcDNA3.1-C-Flag, pcDNA3.1-N-SBP-Flag, pGEX6-GST, and the biomolecular complementation affinity purification system vectors pDEST-V1-ORF, pDEST-V2-ORF, pDEST-ORF-V1 and pDEST-ORF-V2 using a LR Clonase Reaction kit (Invitrogen). ..

    Article Title: Heteromeric clusters of ubiquitinated ER-shaping proteins drive ER-phagy.
    Article Snippet: .. Plasmids are presented in Supplementary Table 1. cDNAs were cloned into the pDONR223 vector using a BP Clonase Reaction kit (Invitrogen, 11789100) and further recombined into the Gateway destination vectors pcDNA5-FRT/TO-N-mCherry-EGFP, pcDNA3.1-N-HA, pHAGE-GFP, pcDNA3.1-N-Flag, pcDNA3.1-C-Flag, pcDNA3.1-N-SBP-Flag, pGEX6-GST, and the biomolecular complementation affinity purification system vectors pDEST-V1-ORF, pDEST-V2-ORF, pDEST-ORF-V1 and pDEST-ORF-V2 using a LR Clonase Reaction kit (Invitrogen). ..

    Cloning:

    Article Title: Two FAM134B isoforms differentially regulate ER dynamics during myogenesis
    Article Snippet: cDNAs were cloned into lentiviral vectors using GATEWAY (Thermo Fisher Scientific) or In-Fusion (Takara Bio) systems. .. For the GATEWAY cloning the cDNAs were cloned into the pDONR223 vector using the BP Clonase Reaction Kit (Thermo Fisher Scientific) and further recombined, though the LR Clonase Reaction Kit (Thermo Fisher Scientific), into GATEWAY lentiviral destination vectors: pLTD-N-HA-PUROMYCIN lentiviral vector. .. We designed pLTD-N-HA-BLASTICIDIN and pLTD-N-HAHYGROMYCIN lentiviral vectors that were generated by Vector Builder.

    Generated:

    Article Title: Role of FAM134 paralogues in endoplasmic reticulum remodeling, ER‐phagy, and Collagen quality control
    Article Snippet: .. cDNAs were cloned into pDONR223 vector using the BP Clonase Reaction Kit (Invitrogen) and further recombined, though the LR Clonase Reaction Kit (Invitrogen), into GATEWAY destination vectors: iTAP MSCV‐N‐FLAG‐HA IRES‐PURO, pBABE destination vector (Addgene #51070) and pBABE (Addgene #51070) where we enzymatically changed the resistance from puromycin to hygromycin. cDNA mutations were generated via PCR site‐directed mutagenesis according to standard protocols. ..

    Polymerase Chain Reaction:

    Article Title: Role of FAM134 paralogues in endoplasmic reticulum remodeling, ER‐phagy, and Collagen quality control
    Article Snippet: .. cDNAs were cloned into pDONR223 vector using the BP Clonase Reaction Kit (Invitrogen) and further recombined, though the LR Clonase Reaction Kit (Invitrogen), into GATEWAY destination vectors: iTAP MSCV‐N‐FLAG‐HA IRES‐PURO, pBABE destination vector (Addgene #51070) and pBABE (Addgene #51070) where we enzymatically changed the resistance from puromycin to hygromycin. cDNA mutations were generated via PCR site‐directed mutagenesis according to standard protocols. ..

    Mutagenesis:

    Article Title: Role of FAM134 paralogues in endoplasmic reticulum remodeling, ER‐phagy, and Collagen quality control
    Article Snippet: .. cDNAs were cloned into pDONR223 vector using the BP Clonase Reaction Kit (Invitrogen) and further recombined, though the LR Clonase Reaction Kit (Invitrogen), into GATEWAY destination vectors: iTAP MSCV‐N‐FLAG‐HA IRES‐PURO, pBABE destination vector (Addgene #51070) and pBABE (Addgene #51070) where we enzymatically changed the resistance from puromycin to hygromycin. cDNA mutations were generated via PCR site‐directed mutagenesis according to standard protocols. ..

    Affinity Purification:

    Article Title: Heteromeric clusters of ubiquitinated ER-shaping proteins drive ER-phagy
    Article Snippet: .. Plasmids are presented in Supplementary Table . cDNAs were cloned into the pDONR223 vector using a BP Clonase Reaction kit (Invitrogen, 11789100) and further recombined into the Gateway destination vectors pcDNA5-FRT/TO-N-mCherry-EGFP, pcDNA3.1-N-HA, pHAGE-GFP, pcDNA3.1-N-Flag, pcDNA3.1-C-Flag, pcDNA3.1-N-SBP-Flag, pGEX6-GST, and the biomolecular complementation affinity purification system vectors pDEST-V1-ORF, pDEST-V2-ORF, pDEST-ORF-V1 and pDEST-ORF-V2 using a LR Clonase Reaction kit (Invitrogen). ..

    Article Title: Heteromeric clusters of ubiquitinated ER-shaping proteins drive ER-phagy.
    Article Snippet: .. Plasmids are presented in Supplementary Table 1. cDNAs were cloned into the pDONR223 vector using a BP Clonase Reaction kit (Invitrogen, 11789100) and further recombined into the Gateway destination vectors pcDNA5-FRT/TO-N-mCherry-EGFP, pcDNA3.1-N-HA, pHAGE-GFP, pcDNA3.1-N-Flag, pcDNA3.1-C-Flag, pcDNA3.1-N-SBP-Flag, pGEX6-GST, and the biomolecular complementation affinity purification system vectors pDEST-V1-ORF, pDEST-V2-ORF, pDEST-ORF-V1 and pDEST-ORF-V2 using a LR Clonase Reaction kit (Invitrogen). ..



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